Pomegranate Punica Granatum fruit peels extracts for its free radical scavenging and anti- inflamatory activity

To evaluate free radical scavenging and anti-inflammatory activity of petroleum ether, ethylacetate, aqueous, methanol: water and methanol extracts of Punica granatum fruit peels (Family: Lythraceae) by in vitro methods.All the extracts of Punica granatum fruit peels exhibited significant free radical scavenging effect. The methanol extract exhibited  maximum significant  DPPH  and nitric oxide radical scavenging activity with IC50 value of 24.43 and 45.56µg/ml and  maximum stabilization (86.96%) of HRBC membrane at 80µg/ml among all the extracts of Punica granatum fruit peels. Methanol as an extraction solvent was found to be the best in obtaining the extract of Punica granatum fruit peels rich in radical scavenging and anti-inflammatory phytoconsituents.

pomegranate 43RESEARCH TITLE:  Evaluation of Punica Granatum fruit peels extracts for its free radical scavenging and anti- inflamatory activity

WEB TITLE: Pomegranate Punica Granatum fruit peels extracts for its free radical scavenging and anti- inflamatory activity

COUNTRIES: India

CONDUCTED BY: Minal campus, Bhopal, India

PUBLISHED ON: International Journal of Pharmacy and Pharmaceutical Sciences

RESEARCH:

Abstract

Objective: To evaluate free radical scavenging and anti-inflammatory activity of petroleum ether, ethylacetate, aqueous, methanol:water and methanol extracts of Punica granatum fruit peels (Family: Lythraceae) by in vitro methods.

Methods: The free radical scavenging effect was studied using 1, 1‐Diphenyl‐2‐ Picrylhydrazyl (DPPH) and nitric oxide radical scavenging assay. Anti‐inflammatory activity was evaluated by HRBC membrane stabilization assay.

Results: All the extracts of Punica granatum fruit peels exhibited significant free radical scavenging effect. The methanol extract exhibited  maximum significant  DPPH  and nitric oxide radical scavenging activity with IC50 value of 24.43 and 45.56µg/ml and  maximum stabilization (86.96%) of HRBC membrane at 80µg/ml among all the extracts of Punica granatum fruit peels.

Conclusion: Methanol as an extraction solvent was found to be the best in obtaining the extract of Punica granatum fruit peels rich in radical scavenging and anti-inflammatory phytoconsituents.

RESEACH SUMMARY:

To evaluate free radical scavenging and anti-inflammatory activity of petroleum ether, ethylacetate, aqueous, methanol: water and methanol extracts of Punica granatum fruit peels (Family: Lythraceae) by in vitro methods.All the extracts of Punica granatum fruit peels exhibited significant free radical scavenging effect. The methanol extract exhibited  maximum significant  DPPH  and nitric oxide radical scavenging activity with IC50 value of 24.43 and 45.56µg/ml and  maximum stabilization (86.96%) of HRBC membrane at 80µg/ml among all the extracts of Punica granatum fruit peels. Methanol as an extraction solvent was found to be the best in obtaining the extract of Punica granatum fruit peels rich in radical scavenging and anti-inflammatory phytoconsituents.

YEAR: 2015